High Resolution Thermal Melt Analysis

Detection Technology

High Resolution Thermal Melt (HRM) analysis is a powerful molecular diagnostic technology for detecting mutations in patient DNA samples.

During the HRM process, DNA is heated and the two strands of a DNA molecule separate, or melt apart, at a specific temperature based on the composition of the DNA.  Samples from two normal patient samples yield the same melt curve. However, if one of the samples has a genetic variation, the melting temperature of that DNA will not be the same, thus resulting in a different melt curve, as seen above. 

The key to effective HRM analysis is the optical monitoring system that captures the melting process in real time at high resolution. We are taking HRM analysis to the next level by leveraging Canon-brand industry-leading CMOS sensors for improved melt curve analysis. Using advanced micro-fluid technologies and uniting rapid PCR with HRM, Canon U.S. Life Sciences, Inc. is working to develop a complete molecular diagnostics solution.